Flowcell  AC88UTACXX
Run Start  2015-12-23
Read Length  50 single end
HiSeq Sequencing Kit Trueseq v3

Sequencer Software	HiSeq Control Software	2.2.58
Gerald version     1.8

Alignment Report:

Legend:
Total - total reads considered ( random 10% of total reads sequenced )
Total Unique - total reads aligning to only a single location in the reference
U0 - # of unique hits with 0 mismatches
U1 - # of unique hits with 1 mismatches
U2 - # of unique hits with 2 mismatches
R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches
NM - # of reads without a match to the reference

Mapping Algorithm : GEM_1.7.1 (build 1.819 (beta)) allowing up to 2 mismatches

Lane 1

Sample: Klf 4
Index: CTTGTA
Lab ID: 12372_CTTGTA
Application: ChIP Seq
Reference: M.musculus (GRCm38.dna)

Total	6379910
Total Unique	1808677	28.3%

U0	1693242	26.5%
U1	92220	1.4%
U2	23215	0.4%
R012	324438	5.1%
NM	4140379	64.9%

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Lane 1

Sample: Klf 4
Index: GCCAAT
Lab ID: 12372_GCCAAT
Application: ChIP Seq
Reference: M.musculus (GRCm38.dna)

Total	6036061
Total Unique	1243656	20.6%

U0	1156926	19.2%
U1	69127	1.1%
U2	17603	0.3%
R012	218542	3.6%
NM	4498350	74.5%

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Lane 1

Sample: Klf 4
Index: TTAGGC
Lab ID: 12372_TTAGGC
Application: ChIP Seq
Reference: M.musculus (GRCm38.dna)

Total	4580402
Total Unique	1708332	37.3%

U0	1583701	34.6%
U1	100911	2.2%
U2	23720	0.5%
R012	257133	5.6%
NM	2538839	55.4%

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