Flowcell  AC6BFYANXX
Run Start  2015-02-16
Read Length  50 paired end
HiSeq Sequencing Kit Trueseq v4

Sequencer Software	HiSeq Control Software	2.2.58
Gerald version     1.8

Alignment Report:

Legend:
Total - total reads considered ( random 10% of total reads sequenced )
Total Unique - total reads aligning to only a single location in the reference
U0 - # of unique hits with 0 mismatches
U1 - # of unique hits with 1 mismatches
U2 - # of unique hits with 2 mismatches
R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches
NM - # of reads without a match to the reference

Mapping Algorithm : GEM_1.7.1 (build 1.819 (beta)) allowing up to 2 mismatches

Lane 8 Read 1

Sample: ES 1
Index: GCCAAT
Lab ID: 9273
Application: Smarter-Seq
Reference: M.musculus (GRCm38.dna)

Total	11393231
Total Unique	6175047	54.2%

U0	5357958	47.0%
U1	567578	5.0%
U2	249511	2.2%
R012	2055081	18.0%
NM	2252767	19.8%

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Lane 8 Read 2

Sample: ES 1
Index: GCCAAT
Lab ID: 9273
Application: Smarter-Seq
Reference: M.musculus (GRCm38.dna)

Total	11393231
Total Unique	6037857	53.0%

U0	5163108	45.3%
U1	602562	5.3%
U2	272187	2.4%
R012	1963166	17.2%
NM	2197626	19.3%

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Lane 8 Read 1

Sample: ES 2
Index: CTTGTA
Lab ID: 9274
Application: Smarter-Seq
Reference: M.musculus (GRCm38.dna)

Total	11468188
Total Unique	5804714	50.6%

U0	4952207	43.2%
U1	568627	5.0%
U2	283880	2.5%
R012	2132402	18.6%
NM	2511706	21.9%

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Lane 8 Read 2

Sample: ES 2
Index: CTTGTA
Lab ID: 9274
Application: Smarter-Seq
Reference: M.musculus (GRCm38.dna)

Total	11468188
Total Unique	5635767	49.1%

U0	4731278	41.3%
U1	601897	5.2%
U2	302592	2.6%
R012	2022105	17.6%
NM	2521469	22.0%

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