Flowcell  BC4WCWACXX
Run Start  2014-06-19
Read Length  50 single end
HiSeq Sequencing Kit Trueseq v3

Sequencer Software	HiSeq Control Software	2.0
Gerald version     1.8

Alignment Report:

Legend:
Total - total reads considered ( random 10% of total reads sequenced )
Total Unique - total reads aligning to only a single location in the reference
U0 - # of unique hits with 0 mismatches
U1 - # of unique hits with 1 mismatches
U2 - # of unique hits with 2 mismatches
R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches
NM - # of reads without a match to the reference

Mapping Algorithm : GEM allowing up to 2 mismatches

Lane 2

Sample: B cells K27ac
Index: TTAGGC
Lab ID: 7302
Application: NebNext Ultra ChIP
Reference: M.musculus (mm10)

Total	4295885
Total Unique	2345041	54.6%

U0	2035922	47.4%
U1	230146	5.4%
U2	78973	1.8%
R012	1257700	29.3%
NM	582936	13.6%

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Lane 2

Sample: B cells K9me1
Index: GATCAG
Lab ID: 7303
Application: NebNext Ultra ChIP
Reference: M.musculus (mm10)

Total	5314758
Total Unique	3465265	65.2%

U0	3109347	58.5%
U1	274979	5.2%
U2	80939	1.5%
R012	1277881	24.0%
NM	465500	8.8%

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Lane 2

Sample: input
Index: ACTGAT
Lab ID: 7304
Application: NebNext Ultra ChIP
Reference: M.musculus (mm10)

Total	5727796
Total Unique	2547052	44.5%

U0	2059483	36.0%
U1	347071	6.1%
U2	140498	2.5%
R012	2279297	39.8%
NM	647051	11.3%

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