Flowcell AC6DTUANXX Run Start 2015-04-15 Read Length 50 paired end HiSeq Sequencing Kit Trueseq v4 Sequencer Software HiSeq Control Software 2.2.58 Gerald version 1.8 Alignment Report: Legend: Total - total reads considered ( random 10% of total reads sequenced ) Total Unique - total reads aligning to only a single location in the reference U0 - # of unique hits with 0 mismatches U1 - # of unique hits with 1 mismatches U2 - # of unique hits with 2 mismatches R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches NM - # of reads without a match to the reference Mapping Algorithm : GEM_1.7.1 (build 1.819 (beta)) allowing up to 2 mismatches Lane 345 Read 1 Sample: ATAC lib B Index: TAAGGC Lab ID: 7339 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 13520851 Total Unique 8724395 64.5% U0 7498557 55.5% U1 892841 6.6% U2 332997 2.5% R012 2459972 18.2% NM 1591303 11.8% ------------------------------------------------ Lane 345 Read 2 Sample: ATAC lib B Index: TAAGGC Lab ID: 7339 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 13520851 Total Unique 8645799 63.9% U0 7668080 56.7% U1 600072 4.4% U2 377647 2.8% R012 2522038 18.7% NM 1640875 12.1% ------------------------------------------------ Lane 345 Read 1 Sample: ATAC lib 18 Index: CGTACT Lab ID: 7340 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 15020688 Total Unique 9456319 63.0% U0 8030947 53.5% U1 978523 6.5% U2 446849 3.0% R012 2097018 14.0% NM 2716173 18.1% ------------------------------------------------ Lane 345 Read 2 Sample: ATAC lib 18 Index: CGTACT Lab ID: 7340 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 15020688 Total Unique 9366085 62.4% U0 8149074 54.3% U1 717389 4.8% U2 499622 3.3% R012 2138370 14.2% NM 2768915 18.4% ------------------------------------------------ Lane 345 Read 1 Sample: ATAC lib d1 Index: AGGCAG Lab ID: 7341 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 14025046 Total Unique 9360194 66.7% U0 8016945 57.2% U1 953996 6.8% U2 389253 2.8% R012 1779911 12.7% NM 2282055 16.3% ------------------------------------------------ Lane 345 Read 2 Sample: ATAC lib d1 Index: AGGCAG Lab ID: 7341 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 14025046 Total Unique 9232393 65.8% U0 8062919 57.5% U1 715915 5.1% U2 453559 3.2% R012 1801711 12.8% NM 2333714 16.6% ------------------------------------------------ Lane 345 Read 1 Sample: ATAC lib d2 Index: TCCTGA Lab ID: 7342 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 12207345 Total Unique 7841151 64.2% U0 6902869 56.5% U1 713274 5.8% U2 225008 1.8% R012 2474908 20.3% NM 1162689 9.5% ------------------------------------------------ Lane 345 Read 2 Sample: ATAC lib d2 Index: TCCTGA Lab ID: 7342 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 12207345 Total Unique 7777705 63.7% U0 7051934 57.8% U1 469390 3.8% U2 256381 2.1% R012 2540451 20.8% NM 1204991 9.9% ------------------------------------------------ Lane 345 Read 1 Sample: ATAC Lib E14 Index: GGACTC Lab ID: 7592 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 7247501 Total Unique 4185613 57.8% U0 3604344 49.7% U1 422779 5.8% U2 158490 2.2% R012 1916192 26.4% NM 670396 9.3% ------------------------------------------------ Lane 345 Read 2 Sample: ATAC Lib E14 Index: GGACTC Lab ID: 7592 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 7247501 Total Unique 4143490 57.2% U0 3633382 50.1% U1 329871 4.6% U2 180237 2.5% R012 1937122 26.7% NM 692619 9.6% ------------------------------------------------ Lane 345 Read 1 Sample: GMP ATAC lib Index: TAGGCA Lab ID: 8188 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 8947196 Total Unique 5023048 56.1% U0 4299586 48.1% U1 532774 6.0% U2 190688 2.1% R012 1832995 20.5% NM 1565532 17.5% ------------------------------------------------ Lane 345 Read 2 Sample: GMP ATAC lib Index: TAGGCA Lab ID: 8188 Application: ATACseq Reference: M.musculus (GRCm38.dna) Total 8947196 Total Unique 4973477 55.6% U0 4364726 48.8% U1 394018 4.4% U2 214733 2.4% R012 1867455 20.9% NM 1590137 17.8% ------------------------------------------------