Flowcell BC5L8MANXX Run Start 2014-09-26 Read Length 50 paired end HiSeq Sequencing Kit Trueseq v4 Sequencer Software HiSeq Control Software 2.2.38 Gerald version 1.8 Alignment Report: Legend: Total - total reads considered ( random 10% of total reads sequenced ) Total Unique - total reads aligning to only a single location in the reference U0 - # of unique hits with 0 mismatches U1 - # of unique hits with 1 mismatches U2 - # of unique hits with 2 mismatches R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches NM - # of reads without a match to the reference Mapping Algorithm : GEM allowing up to 2 mismatches Lane 7 Read 1 Sample: ATAC lib B Index: TAAGGCG Lab ID: 7339 Application: ATACseq Reference: M.musculus (mm10) Total 4638463 Total Unique 3054679 65.9% U0 2667930 57.5% U1 279377 6.0% U2 107372 2.3% R012 868058 18.7% NM 542945 11.7% ------------------------------------------------ Lane 7 Read 2 Sample: ATAC lib B Index: TAAGGCG Lab ID: 7339 Application: ATACseq Reference: M.musculus (mm10) Total 4638463 Total Unique 2849490 61.4% U0 2329245 50.2% U1 360275 7.8% U2 159970 3.4% R012 814339 17.6% NM 689404 14.9% ------------------------------------------------ Lane 7 Read 1 Sample: ATAC lib 18 Index: CGTACTA Lab ID: 7340 Application: ATACseq Reference: M.musculus (mm10) Total 6120287 Total Unique 3924478 64.1% U0 3380923 55.2% U1 384314 6.3% U2 159241 2.6% R012 891974 14.6% NM 1079358 17.6% ------------------------------------------------ Lane 7 Read 2 Sample: ATAC lib 18 Index: CGTACTA Lab ID: 7340 Application: ATACseq Reference: M.musculus (mm10) Total 6120287 Total Unique 3680094 60.1% U0 2968092 48.5% U1 486452 7.9% U2 225550 3.7% R012 838493 13.7% NM 1256279 20.5% ------------------------------------------------ Lane 7 Read 1 Sample: ATAC lib d1 Index: AGGCAGA Lab ID: 7341 Application: ATACseq Reference: M.musculus (mm10) Total 6580566 Total Unique 4457913 67.7% U0 3880292 59.0% U1 411979 6.3% U2 165642 2.5% R012 871881 13.2% NM 1053799 16.0% ------------------------------------------------ Lane 7 Read 2 Sample: ATAC lib d1 Index: AGGCAGA Lab ID: 7341 Application: ATACseq Reference: M.musculus (mm10) Total 6580566 Total Unique 4099861 62.3% U0 3265765 49.6% U1 570067 8.7% U2 264029 4.0% R012 799321 12.1% NM 1269940 19.3% ------------------------------------------------ Lane 7 Read 1 Sample: ATAC lib d2 Index: TCCTGAG Lab ID: 7342 Application: ATACseq Reference: M.musculus (mm10) Total 4508704 Total Unique 2989110 66.3% U0 2639373 58.5% U1 259051 5.7% U2 90686 2.0% R012 936139 20.8% NM 420222 9.3% ------------------------------------------------ Lane 7 Read 2 Sample: ATAC lib d2 Index: TCCTGAG Lab ID: 7342 Application: ATACseq Reference: M.musculus (mm10) Total 4508704 Total Unique 2819517 62.5% U0 2351362 52.2% U1 331908 7.4% U2 136247 3.0% R012 886366 19.7% NM 553335 12.3% ------------------------------------------------ Lane 8 Read 1 Sample: ATAC Lib E14 Index: GGACTCC Lab ID: 7592 Application: ATACseq Reference: M.musculus (mm10) Total 8035078 Total Unique 5032269 62.6% U0 4568889 56.9% U1 352290 4.4% U2 111090 1.4% R012 2098672 26.1% NM 808528 10.1% ------------------------------------------------ Lane 8 Read 2 Sample: ATAC Lib E14 Index: GGACTCC Lab ID: 7592 Application: ATACseq Reference: M.musculus (mm10) Total 8035078 Total Unique 4949022 61.6% U0 4386300 54.6% U1 406405 5.1% U2 156317 1.9% R012 2069279 25.8% NM 847430 10.5% ------------------------------------------------ Lane 8 Read 1 Sample: BII ATAC lib Index: CTCTCTA Lab ID: 8187 Application: ATACseq Reference: M.musculus (mm10) Total 8785954 Total Unique 5797632 66.0% U0 5219204 59.4% U1 434346 4.9% U2 144082 1.6% R012 1597434 18.2% NM 1283406 14.6% ------------------------------------------------ Lane 8 Read 2 Sample: BII ATAC lib Index: CTCTCTA Lab ID: 8187 Application: ATACseq Reference: M.musculus (mm10) Total 8785954 Total Unique 5718636 65.1% U0 5035175 57.3% U1 487798 5.6% U2 195663 2.2% R012 1579383 18.0% NM 1332515 15.2% ------------------------------------------------ Lane 8 Read 1 Sample: GMP ATAC lib Index: TAGGCAT Lab ID: 8188 Application: ATACseq Reference: M.musculus (mm10) Total 9714690 Total Unique 5827324 60.0% U0 5283858 54.4% U1 415488 4.3% U2 127978 1.3% R012 2013233 20.7% NM 1767435 18.2% ------------------------------------------------ Lane 8 Read 2 Sample: GMP ATAC lib Index: TAGGCAT Lab ID: 8188 Application: ATACseq Reference: M.musculus (mm10) Total 9714690 Total Unique 5736054 59.0% U0 5074107 52.2% U1 479982 4.9% U2 181965 1.9% R012 1985420 20.4% NM 1805999 18.6% ------------------------------------------------