Flowcell BC4WCWACXX Run Start 2014-06-19 Read Length 50 single end HiSeq Sequencing Kit Trueseq v3 Sequencer Software HiSeq Control Software 2.0 Gerald version 1.8 Alignment Report: Legend: Total - total reads considered ( random 10% of total reads sequenced ) Total Unique - total reads aligning to only a single location in the reference U0 - # of unique hits with 0 mismatches U1 - # of unique hits with 1 mismatches U2 - # of unique hits with 2 mismatches R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches NM - # of reads without a match to the reference Mapping Algorithm : GEM allowing up to 2 mismatches Lane 6 Sample: K27 Index: CGATGT Lab ID: 7299 Application: NebNext Ultra ChIP Reference: M.musculus (mm10) Total 4364999 Total Unique 3298808 75.6% U0 2998914 68.7% U1 244087 5.6% U2 55807 1.3% R012 787246 18.0% NM 215909 4.9% ------------------------------------------------ Lane 6 Sample: IgG Index: CAGATC Lab ID: 7300 Application: NebNext Ultra ChIP Reference: M.musculus (mm10) Total 5886751 Total Unique 4298195 73.0% U0 3883165 66.0% U1 335719 5.7% U2 79311 1.3% R012 1169905 19.9% NM 298211 5.1% ------------------------------------------------ Lane 6 Sample: IN Index: GTGAAA Lab ID: 7301 Application: NebNext Ultra ChIP Reference: M.musculus (mm10) Total 7802128 Total Unique 6004190 77.0% U0 5467925 70.1% U1 439945 5.6% U2 96320 1.2% R012 1389314 17.8% NM 284495 3.6% ------------------------------------------------