Abstract

The RNA-seq data used for the analysis comes from samples of a human lung epithelial adenocarcinoma cell line (A549). Samples were treated with 100 nM of dexamethasone, a synthetic glucocorticoid.

Polyadenylated mRNA was collected from treated samples at two different time intervals following treatment initiation, and from control samples. For each time point there are 4 biological replicates. Hence, in total we have 12 samples, 4 samples for 0 hours (control), 4 samples for 2 hours after treatment, and 4 samples for 4 hours after treatment.

Paired-end RNA sequencing was conducted to examine the effect of dexamethasone on human cells.

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