FastQCFastQC Report
Tue 7 Oct 2025
HCM-10.fastp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM-10.fastp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773691
Total Bases16.7 Mbp
Sequences flagged as poor quality0
Sequence length17-41
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG14312318.498728820679055No Hit
TGAGGTAGTAGGTTGTGTGGT515376.661186442649584No Hit
TGAGGTAGTAGGTTGTATAG439325.678235884868766No Hit
TGAGGTAGTAGGTTGTATAGTT382104.93866414369561No Hit
TGAGGTAGTAGGTTGTGTGGTT367574.750863070657408No Hit
TGAGGTAGTAGTTTGTACAGT253313.2740460985070268No Hit
TGAGGTAGTAGGTTGTATAGT228252.9501441790068648No Hit
TGAGGGGCAGAGAGCGAGACTTT202902.6224939930799245No Hit
TGAGGTAGTAGGTTGTGTGG164902.1313418406056166No Hit
TGAGGTAGTAGTTTGTGCTGTT162672.102518964289361No Hit
TGAGGTAGTAGATTGTATAGT130381.6851688852526396No Hit
TGAGGTAGTAGATTGTATAGTT114591.4810822408429205No Hit
TCCTGTACTGAGCTGCCCCGAG99611.2874648923148906No Hit
TAGCAGCACGTAAATATTGGC92201.1916902225824004No Hit
TGAGGTAGTAGTTTGTACAGTT85411.103929087969228No Hit
TGAGGGGCAGAGAGCGAGACT85061.0994053181438068No Hit
TAGCAGCACGTAAATATTGG83871.0840245007373743No Hit
TCGAGGAGCTCACAGTCTAGT73730.9529644263665985No Hit
TTCAAGTAATCCAGGATAGGCT65640.8484007181161471No Hit
TCGTACCGTGAGTAATAATGCG60730.7849386899938089No Hit
TCCTGTACTGAGCTGCCCCGAGA49710.6425045657762595No Hit
TCCTGTACTGAGCTGCCCCGA49300.6372052925521946No Hit
CGTACCGTGAGTAATAATGCG46590.60217838904679No Hit
TGAGGTAGTAGTTTGTGCTGT44620.5767160274579903No Hit
TGAGGTAGTAGATTGTATAG40650.5254035525810692No Hit
TGGAGTGTGACAATGGTGTTT38800.5014921977895568No Hit
TGAGGTAGTAGTTTGTACAG36930.47732234186516326No Hit
TTGAGGATATGAGATCGAGACGACA35820.4629755289902558No Hit
TCGAGGAGCTCACAGTCTAGTA34360.4441049462899271No Hit
TGAGGTAGTAGTTTGTGCT33980.439193424765184No Hit
TGAGGTAGTAGGTTGTGTGGTTT33800.43686691456925314No Hit
TGAGGTAGTAGATTGTATAGTTT27270.35246629468353646No Hit
TGTAAACATCCTTGACTGGAAGCT22130.2860315035330642No Hit
TGAGGTAGTAGTTTGTGCTG20350.26302490270663614No Hit
CAAAGTGCTGTTCGTGCAGGTAG20330.2627664015737549No Hit
TGGAGAGAAAGGCAGTTCCTGA20240.26160314647578947No Hit
TGAGGTAGTAGGTTGTATA19090.24673933133511958No Hit
TGAGGTAGTAGGTTGTATAGTTT18840.2435080671741044No Hit
AGAGGTAGTAGGTTGCATAGT17970.23226326789377152No Hit
TGAGGTAGTAGGTTGTGTGGTTAT17680.22851500146699394No Hit
TGAGGTAGTAAGTTGTATTGTT16830.2175287033195423No Hit
TCGAGGAGCTCACAGTCTAG16570.21416818859208653No Hit
GGAGGTAGTAGGTTGTGTGGT16550.2139096874592053No Hit
TTCAAGTAATTCAGGATAGGTT16100.20809341196937797No Hit
TCGAGGAGCTCACAGTCTAGTAA16070.20770566027005613No Hit
TGAGGGGCAGAGAGCGAGACTT15880.20524989950768457No Hit
TTGCATAGCTGGAGAAGTGATC15790.20408664440971913No Hit
CAAAGTGCTGTTCGTGCAGGT15170.19607310929040145No Hit
TGAGGTAGTAAGTTGTATTGT14750.19064458549989596No Hit
AGAGGTAGTAGGTTGCATAG14280.1845698088771874No Hit
TGAGGTAGTAGGTTGTGTGGTA13980.18069229188396918No Hit
TGAGGGGCAGAGAGCGAGA13950.18030454018464734No Hit
GGAGGTAGTAGGTTGTATAG13950.18030454018464734No Hit
GGTGGTAGTAGGTTGTGTGGT13860.1791412850866819No Hit
TTCAAGTAATTCAGGATAGGT13750.17771952885583522No Hit
TCCTGTACTGAGCTGCCCCG13720.17733177715651338No Hit
TGTAAACATCCCCGACTGGAAGCT13100.16931824203719573No Hit
GGTGGTAGTAGGTTGTATAG12870.16634547900906174No Hit
TGAGGTAGTAGGTTGTGTG12610.16298496428160594No Hit
GGAGGTAGTAGGTTGTATAGTT12320.15923669785482833No Hit
GGAGGTAGTAGGTTGTGTGGTT12200.15768569105754107No Hit
AGAGGTAGTAGGTTGCATAGTT12180.15742718992465984No Hit
TCCTGTACTGAGCTGCCCCGAGT12170.15729793935821923No Hit
GAGCAGCACGTAAATATTGGCG12090.15626393482669437No Hit
AAAAGCTGGGTTGAGAGGGCGA11830.15290342009923857No Hit
AGCAGCACGTAAATATTGGCG11640.15044765933686705No Hit
GGTGGTAGTAGGTTGTATAGTT11230.14514838611280215No Hit
TGAGGTAGTAGGTTGTGTGGTTA11030.14256337478399No Hit
GGTGGTAGTAGGTTGTGTGGTT10920.1411416185531433No Hit
CAAAGTGCTGTTCGTGCAGGTA10720.13855660722433116No Hit
TGGAGTGTGACAATGGTGTTTGA10340.13364508569958808No Hit
AGTTGGTCCGAGTGTTGT10210.13196482833586018No Hit
TCATGGTCAGATCCGTCATCC10030.12963831813992924No Hit
TGAGGGGCAGAGAGCGAGAC9980.12899206530772622No Hit
CATTATTACTTTTGGTACGCG9410.12162478302061158No Hit
CAACGGAATCCCAAAAGCAGCT9230.11929827282468065No Hit
AAAAGCTGGGTTGAGAGGGCG8930.11542075583146243No Hit
TGGAGTGTGACAATGGTGTTTG8440.10908747807587267No Hit
GGAGGTAGTAGTTTGTACAGT8280.10701946901282296No Hit
TGAGGTAGTAGTTTGTACAGTTA8120.10495145994977323No Hit
TTCAAGTAATCCAGGATAGGCTT8020.10365895428536716No Hit
TGAGGTAGTAGTTTGTGCTGTTA7810.1009446923901144No Hit
TGAGGTAGTAGATTGTATA7810.1009446923901144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)