FastQCFastQC Report
Fri 13 Jan 2012
5T_HEX6GYL01_Amplicons_signalProcessing.fastq

Summary

[FAIL] Basic Statistics

Measure Value
Filename 5T_HEX6GYL01_Amplicons_signalProcessing.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 417922
Sequence length 40-1596
%GC 43

[FAIL] Per base sequence quality

Per base quality graph

[FAIL] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[FAIL] Per base N content

N content graph

[FAIL] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGTACGCG 114028 27.28451720656008 No Hit
ACCAACGCCAAGAATTAGAGGAGCTGGAAGCAGACTTAAAGAAACGGGAG 4648 1.112169256464125 No Hit
CTAATACGACTCACTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGT 4095 0.9798479142040859 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGTACGTC 3555 0.8506372002431076 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGTACTTT 3325 0.7956030072597278 No Hit
CGGATTGCAGTCAAGTCCACTGAAGTAGAATAAGGAGACAGGCATCCAGG 2443 0.5845588411234632 No Hit
CTCGGATCAAAACTTTCCCGAGGAAATTTGACAATTTTCTGTGATTTTAT 1975 0.47257622235728197 No Hit
AGTAGGACACCACTTCCAGCAAGAGCAAAAGATGTGTCTTTTTCCTCATG 1925 0.46061226736089506 No Hit
CTAATACGACTCACTATAGGGCTCAGGCGTTTGTGTATGATATGTTTGCG 1829 0.43764147376783225 No Hit
AGTAAGGCTTTAAAGCTCCTGCACGGCGTGCAGTTTTCTGTGTTTGTGTG 1737 0.41562779657448046 No Hit
CGGATTGCAGTCAAGTCCACTGAAGTAGGATAAGGAGACAGGCATCCAGG 1623 0.3883499791827183 No Hit
ATCAACACCTCCATCGGCACGGAATTAGCTCGTTTTCCAGACCGCCATGA 1543 0.3692076511884993 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGTATCACGCAGAGTACGCGG 1283 0.3069950852072875 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGACTTTT 1155 0.27636736041653703 No Hit
CCCTGTTTCTTGCCAACACCTGGTTAGTCTGATTAATTTTAGTCAGTTTA 1093 0.26153205622101733 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGTATAACGCAGAGTACGCGG 1071 0.25626791602260707 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGTATCAACGCAGAGTACGCGG 1035 0.24765386842520853 No Hit
AAGTGGTGAACTTAGGCATCCTGGCTCCGGGTCATCATCCTCCGAGGAGA 906 0.21678686453453036 No Hit
GAGGGACAAGGTAATTGAGGATGAGGCCATAAACAGCCTCAACTTAGAGA 830 0.1986016529400223 No Hit
TGGTGACTCCAATTAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA 807 0.19309823364168432 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAAGCAGAGTACGCGG 789 0.18879120984298506 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGTACGTT 721 0.1725202310478989 No Hit
GAAGTCTTCAGGCATGCATGGCTCTCTCTTCATGCTTCCTGGTGGGTCCC 713 0.170605998248477 No Hit
GCGTTCACTGTAGGCCTGAATGCTGATAAAGCTAACCATTTTGGGGTGGT 658 0.15744564775245143 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGTACAACGCAGAGTACGCGG 642 0.15361718215360762 No Hit
AATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGTACGCGGG 639 0.1528993448538244 No Hit
CTAATACGACTCACTATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACG 632 0.15122439115433023 No Hit
CTAATACGACTCACTATAGGGCAGCAGTGGTATCAACGCAGAGTACGCGG 588 0.14069611075750976 No Hit
CATATCCAGAATTATCCCGCTCCCTGGCGTAGATCGGCTTCTGCTGCCTC 562 0.1344748541593886 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGTTCAACGCAGAGTACGCGG 549 0.131364225860328 No Hit
AGCTGCATTGAGTTAGCGGGGGCAGGCCTCCTAGGGAGAGGAGGGTGGAT 542 0.12968927216083384 No Hit
CGTCACCACCTCCCTCAGCATACAGTTAGCCCGTTTTCCAAACTGCCATG 538 0.12873215576112287 No Hit
GTTCATTGCAAGAGGGTTGGTGTGCCTAGTGTGGGAGTACTTGGTCTGCT 538 0.12873215576112287 No Hit
TAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGTACGCGG 537 0.12849287666119516 No Hit
CAGCAGACCATGTGACTACTGCAGTTGAGAAATCCAAGGAAAGTCAAGTG 476 0.11389685156560315 No Hit
CTAATACGACTCACTATAGGGCAAGCATGGTATCAACGCAGAGTACGCGG 473 0.11317901426581994 No Hit
TATTTGAAGCAGAGGTGGGGCTTCTGTAACGAAAGAGAAAGGAGGAAAAT 468 0.11198261876618125 No Hit
CTAATACGACTCACTATAGGGCAAGCAGTGGATCAACGCAGAGTACGCGG 465 0.11126478146639804 No Hit
ATTTCCAGAGGAAGGGACCACTTTGGCTTGGTTCGTAAAAAGAGGCAGCA 457 0.10935054866697613 No Hit
AGTTGTGTATTGCTGGGGAGGCCACCGTGAGGCAAATGCTCAGTTTTCGG 443 0.10600064126798779 No Hit
CCAGTGTTAAATGACTGCCACACAGGGTCTGAGTGCTTGCCAGACTTGGC 441 0.10552208306813234 No Hit
CTAATACGACTCACTATAGGGCAAGCAGGGTATCAACGCAGAGTACGCGG 427 0.102172175669144 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGAGG 581240 2.5508902 7.523727 15-19
TTTTT 1559745 2.5310369 11.600364 50-59
AAAAA 1509305 2.4927402 9.323015 1400-1499
CCTCC 507970 2.4790454 9.352761 8
CAGAG 680220 2.4031832 80.78036 40-44
CCAGG 504660 2.339676 5.383005 45-49
CCTGG 504470 2.3305647 5.1611896 90-99
AGCAG 655655 2.3163965 89.75112 20-24
CTGGG 512305 2.302736 5.8099647 10-14
CAGTG 631200 2.2221503 87.768974 25-29
GCTGG 489235 2.1990397 8.262544 20-24
GGGAG 479910 2.1061828 12.116787 50-59
GGCTG 466155 2.0952985 7.5257335 20-24
AGGGC 461305 2.080821 141.27472 15-19
CAGGC 441390 2.0463471 5.13441 35-39
GCAGA 577385 2.0398722 87.91077 35-39
GGGCA 450760 2.0332556 122.28088 15-19
GCCTG 438305 2.0248938 13.5817795 1
CTCAC 540315 2.0094354 114.160774 10-14
AGTGG 569195 1.9496529 85.13489 25-29
TGCTG 552290 1.9375043 5.65888 10-14
CTGCC 404400 1.9202037 5.4985743 1
CTCCA 516145 1.9195471 14.921681 9
GAGGC 419465 1.8920921 5.488826 15-19
TGTGT 706945 1.8832618 13.393021 4
CTGGA 530125 1.8663142 5.3230495 20-24
GTGGT 533255 1.8201205 84.84085 25-29
GCAGT 516080 1.8168683 85.74486 25-29
TGGGG 407510 1.782147 7.899591 7
TCACT 618615 1.7470171 87.92395 10-14
TGCAG 494760 1.7418112 14.168349 6
CCTGC 362830 1.7228178 5.014989 15-19
AAAAC 792285 1.7171205 13.313629 1500-1591
TCTCT 607895 1.7107016 5.439007 6
GGAGA 497355 1.7095969 5.816706 50-59
CTGAG 485015 1.7075036 10.134482 3
TAGGG 495085 1.6958053 107.206215 15-19
AAGCA 606255 1.677582 69.18799 20-24
CCCTG 351410 1.6685925 5.8470755 1
GGCCA 357130 1.655706 7.1157527 20-24
GGGGG 295255 1.6544081 30.328264 50-59
TCTTT 770770 1.6471004 5.1583714 8
TTTTA 1005785 1.637876 9.828045 1500-1591
GGAAA 608130 1.6372482 5.281393 1500-1591
GGGGT 371880 1.6263279 19.603151 1300-1399
AGGCC 349635 1.620958 7.9025493 15-19
CACAC 430400 1.6063136 6.2240314 2
GGGGA 363705 1.5961934 14.2582245 50-59
GACTC 437970 1.5847514 551.24225 8
CCTTT 556980 1.5674195 5.060269 1500-1591
CACCA 419685 1.5663236 7.213267 8
GGCAA 437820 1.5467962 89.339096 20-24
ACACA 542050 1.5416226 6.2738132 7
GCAAG 435605 1.5389706 88.6303 20-24
AGGCT 435605 1.5335549 12.689636 5
TAAAA 927755 1.5268706 9.932537 1500-1591
GCCAC 315990 1.505707 6.0622807 20-24
ACTCA 526770 1.4928932 431.1039 9
TGGTG 436700 1.4905562 6.499466 1
AGAGT 552865 1.483222 64.96143 40-44
CAAGC 407050 1.4780719 90.43716 20-24
CCTGA 407995 1.4762897 10.829371 2
CTTTC 519670 1.462424 6.609426 9
CACCT 392660 1.4603053 6.4599953 6
GTTTT 701095 1.4576788 10.642549 1500-1591
CCCTT 388620 1.4401947 5.229889 1400-1499
TTAAA 877225 1.4386294 18.480022 1500-1591
TTTGG 537715 1.4324425 5.050896 5
GGGCT 317700 1.4280151 15.52505 15-19
GGGAA 414805 1.4258415 5.3790417 50-59
GGCTC 303315 1.4012634 6.005629 20-24
AAACC 492630 1.401069 27.956913 1500-1591
ACCCC 280675 1.3746152 22.023283 1500-1591
GGGCC 232150 1.3741516 5.3682756 15-19
GCTCC 289155 1.3729883 7.2610846 15-19
GTACG 388175 1.3665769 80.955605 40-44
TTGGG 399525 1.3636694 6.207198 6
ACGAC 374185 1.3587332 553.36664 6
ACTCC 362520 1.3482146 8.977271 8
TTTAA 822850 1.3447068 19.39451 1500-1591
ACCAC 359355 1.3411636 7.289356 9
GGGTG 304620 1.3321824 5.0347867 50-59
GGGGC 231075 1.3307873 10.191567 9
ACCTC 355880 1.3235204 6.278024 7
CCAAG 364185 1.3224212 16.257198 8
CTCTT 468640 1.3188183 5.370158 7
TGGTA 489855 1.3095547 65.70016 25-29
CCCAA 350320 1.3074439 6.756651 4
AAGGG 380300 1.3072346 5.880659 1100-1199
AGTAC 472915 1.304009 66.78338 40-44
GTAGG 375685 1.2868267 6.578207 2
AGTAG 472880 1.2686391 5.414869 1
CCCCC 196315 1.2616832 15.871988 1400-1499
AGGGT 367895 1.2601438 6.080069 1500-1591
ACCCA 336305 1.2551377 6.7566495 3
GCGGG 216580 1.2473089 129.68411 45-49
CACTA 436930 1.2382821 87.91089 10-14
GTCTC 336885 1.2146956 6.2042403 5
GGTAG 353705 1.211539 5.3657036 1000-1099
GGGTA 352955 1.2089701 17.09109 1400-1499
GGTTT 453060 1.2069265 17.651785 1500-1591
GGGTT 353080 1.2051423 25.446424 1500-1591
AGCAA 434005 1.2009451 7.5821667 6
TTTAC 551550 1.1827996 7.638496 1200-1299
AATAC 547670 1.1827891 329.94324 3
TCAAC 417275 1.1825788 68.55018 30-34
AAGGC 334680 1.1824076 12.446339 4
CAAGA 426910 1.1813123 14.250411 9
AGGAC 334065 1.1802349 6.727361 4
CAAGG 333645 1.1787511 5.690235 7
AACCC 315595 1.1778451 25.767855 1500-1591
AACCT 414430 1.1745158 8.106871 1500-1591
GAGTA 436470 1.1709584 64.94611 40-44
GCACA 321515 1.1674789 5.892794 2
CAACA 409750 1.1653535 8.349623 8
ACTAT 540815 1.1638743 66.93707 10-14
GTAAA 551495 1.1588298 8.95426 1300-1399
GGGAC 255615 1.1530095 5.259744 50-59
CGGGG 200055 1.1521394 62.283924 45-49
CGCAG 247955 1.1495548 114.96465 35-39
TGAGC 325385 1.1455235 9.890451 4
CCAAC 306540 1.1440506 16.830755 2
ATAGG 425995 1.1428564 83.34604 15-19
GGTGA 333230 1.1414064 5.2374253 2
ACCTT 401670 1.1343473 6.3065505 1500-1591
TTGGT 424690 1.1313503 5.656706 1500-1591
GAGCA 317855 1.1229658 9.882163 5
TGGTT 420695 1.1207079 22.143934 1500-1591
GCCAA 306635 1.113447 16.33123 7
GGCTT 315665 1.1073934 12.273216 6
GCTTT 403210 1.1039938 9.478478 7
CTTTA 511475 1.0968589 7.1877627 8
CTAAT 508400 1.0941148 329.46176 1
ACCAA 382315 1.0873266 13.104266 1
AGACC 297945 1.081892 6.366663 8
TAAAC 495180 1.0694277 17.22038 1500-1591
GGACA 302525 1.0688057 7.447636 5
CAGTC 291870 1.0561029 13.750809 8
ATCAA 485790 1.0491483 53.011505 30-34
TACCC 281400 1.0465287 13.68322 1400-1499
GGTAT 391385 1.0463097 63.089104 30-34
AGTCA 378795 1.0444839 11.366957 9
TTACC 367125 1.0367895 13.056303 1300-1399
CTATA 480445 1.0339534 66.67732 10-14
GACCA 282660 1.0263895 6.3962755 9
AGTAA 487595 1.0245599 7.6425543 1
CGACT 282665 1.0227954 551.28644 7
ACACC 271715 1.0140787 7.000217 7
TAATA 618165 1.0137768 251.00153 2
TTGTG 379405 1.0107136 13.316986 3
CGCGG 170645 1.0100887 133.03853 45-49
GAGTC 286875 1.0099484 6.1438503 3
TATAG 479520 1.0040463 63.949875 15-19
CCCCG 160335 1.0025704 30.338316 1500-1591
GGTAC 281320 0.99039185 11.104713 1100-1199
GGTAA 368370 0.98826027 13.319865 1300-1399
GTTGT 363010 0.9670383 13.262675 2
GGGAT 280530 0.96089405 6.768372 50-59
GTACC 263385 0.9530328 9.300908 1400-1499
CAACC 255225 0.95253575 11.999573 1400-1499
TAGGA 354525 0.9511171 5.1850853 3
AGTTG 354100 0.94663393 13.636692 1
TATCA 431390 0.9283834 53.06715 30-34
GTATC 335275 0.921229 67.11313 30-34
CAACG 253175 0.91932404 82.54018 35-39
GACAC 251690 0.9139318 10.201467 6
CCCGT 191315 0.90841687 16.702826 1300-1399
AACAT 420615 0.9083916 5.7591934 9
GTGTT 340790 0.90784544 5.0835433 1
GTTAC 328775 0.90336895 13.05597 1200-1299
GTGAC 255935 0.90102357 7.88078 3
GTTTA 431750 0.90084136 13.788607 1500-1591
AAACG 324515 0.89797276 15.295963 1300-1399
CGTAC 248110 0.8977616 9.279555 1000-1099
GTATT 430265 0.89774305 9.988028 7
ACGCA 245120 0.8900749 89.84569 35-39
TTGCA 320475 0.8805632 10.99071 5
AACGC 240780 0.87431556 88.42427 35-39
GTAAG 322510 0.8652275 9.593502 2
TACGA 313060 0.86322695 420.4824 5
TACTC 304855 0.8609343 6.8170114 7
CCCGG 141225 0.8591872 36.935043 1500-1591
TACGT 312295 0.8580872 9.499411 1000-1099
TGTAT 405115 0.8452677 10.15818 6
TAAGG 315025 0.8451468 9.648197 3
GGATT 315045 0.8422261 10.355476 2
GGTTA 313380 0.83777493 17.571936 1300-1399
ACGCG 172705 0.80068505 102.60644 45-49
ACGGG 177330 0.79988724 24.934175 1300-1399
CGGGA 176985 0.798331 33.37636 45-49
GGACG 176555 0.7963914 37.49525 1500-1591
ACGTA 287545 0.7928722 7.9391003 1000-1099
GTCGT 226010 0.79287213 5.490606 900-999
GTAAC 287490 0.79272074 7.8704863 1300-1399
GTTAA 368505 0.7715968 14.418906 1500-1591
GATCA 276820 0.76329947 5.1156316 5
TAACC 268210 0.7601209 11.067327 1500-1591
GTGTA 284240 0.7598735 12.982497 5
CCGGG 128150 0.7585506 40.627026 1500-1591
ATACG 272985 0.7527247 420.76346 4
GCAAC 206390 0.7494393 9.742475 7
TTAAC 343745 0.73976487 15.883316 1500-1591
ATACT 342605 0.73731154 5.2387447 6
TACGC 202260 0.7318579 73.570206 45-49
GGATC 206870 0.72828937 5.899819 4
AACGT 259600 0.71581715 13.883002 1500-1591
ACCCG 148245 0.7063943 19.69334 1400-1499
CGGGT 157065 0.7059842 27.052168 1500-1591
AACGG 197860 0.6990295 22.582935 1300-1399
GACGG 153550 0.6926221 5.424509 1000-1099
ACGGT 194870 0.68604314 14.792024 1100-1199
GATTG 255830 0.68392366 10.300976 3
ATTGC 248880 0.683843 13.018717 9
TATTG 320735 0.66920996 9.971012 8
CGACG 143280 0.6642666 5.8163586 1000-1099
ACCGT 183315 0.66330725 12.677095 1200-1299
CCGTT 183625 0.6620908 17.627708 1400-1499
ACGTT 238105 0.6542367 12.756255 1500-1591
ACGCC 136545 0.6506433 22.20771 5
CGTCG 139800 0.64585197 5.011524 900-999
CGGGC 107700 0.6375021 16.80985 45-49
CCGTA 172510 0.6242105 8.8007145 1300-1399
TACGG 174620 0.61475277 15.25401 1100-1199
CGTTT 218505 0.5982693 15.228399 1500-1591
GCCCG 97250 0.5916512 101.143005 1500-1591
GATAA 270160 0.56767416 5.397768 1
TTACG 200530 0.5509925 11.157553 1100-1199
CCGGT 115305 0.5326893 18.627483 1400-1499
CGTAA 191330 0.5275704 7.3239217 1100-1199
TACCG 143995 0.52103174 12.412991 1200-1299
CTACG 143530 0.5193492 5.1814747 1500-1591
CGGTA 146295 0.5150341 10.740994 1200-1299
CGGTT 146750 0.51481783 18.859562 1500-1591
CTCGG 110210 0.5091513 7.534968 1
AACCG 138685 0.5035902 21.844633 1500-1591
CCGGA 105845 0.49071258 5.9464192 1500-1591
CGCCA 102855 0.49010882 21.0422 6
ACCGG 104785 0.48579824 21.029924 1400-1499
CGTTA 173780 0.4774921 12.374091 1200-1299
GGCGC 71060 0.4206212 5.840862 7
TAACG 149055 0.411002 13.325021 1500-1591
GACGT 115965 0.4082568 29.26417 1500-1591
TCGGA 84330 0.29688522 5.756271 2
CGGAT 66300 0.23341027 13.924314 1
Produced by FastQC (version 0.9.3)