FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_76_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_76_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24727786
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT538690.21784805158051754No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG356840.14430729868011638No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCGAGCTTATGACCCGC321150.12987414239188255No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCACGTGTAAGT306240.12384448813977926No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT289780.11718800866361428No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA278050.11244435712926343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA14950.042.755851
TCACCCG134950.040.678773
TAACGCG55850.032.904217
AACGCGC57450.031.9878148
AGACGCG26650.031.2157574
GCCGCAG94250.031.0673731
CTCACCC177800.030.875142
CGCAGGC94850.030.8212973
TCTAACG61550.030.0861075
CTAACGC64050.028.9851656
GCGGTGT128800.027.1125761
CGGTGTG129300.026.0262952
GAGTCTA71650.025.8450782
GTCTAAC80500.023.0037274
CCTCACC245700.022.6296271
TAGCATA134950.022.0111148
AGTCTAA85500.021.713453
CGGACAC170400.021.58303812-13
GTAGCAT138850.021.325177
GCCCTCT171000.020.5315781
Produced by FastQC (version 0.11.5)