FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_76_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_76_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24727786
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT520360.21043533780177487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG171150.037.5206383
AGACGCG37600.037.4696434
CTCACCC182250.035.6991652
CGCTCGA12700.035.1289441
TAACGCG79450.034.3819057
AACGCGC79050.033.853928
TCTAACG85250.031.9861095
CTAACGC86950.031.5272526
CCTCACC209450.031.0853181
GAGTCTA89550.030.929882
AGTCTAA101950.027.0280443
GTCTAAC105000.026.1964654
TCAGACG55900.026.1045042
GCGGTGT130050.024.0850241
GCCCTCT123100.023.7459851
CGGTGTG133950.022.6552072
CGCAGGC62700.022.2747673
CACCCGG292750.021.770824
GCCGCAG72800.021.3850881
CGGACAC199100.021.34296612-13
Produced by FastQC (version 0.11.5)