FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_75_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_75_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19267956
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT392790.20385660004621142No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT225810.11719457943541078No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCACGTGTAAGT217900.11308931782904218No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG207520.10770213508895286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA11250.037.182221
TCACCCG98200.036.0875743
TAACGCG37000.030.9945937
GCCGCAG74100.030.1282021
AGACGCG16000.029.9624984
AACGCGC38750.029.5948378
CGCAGGC75100.029.2263643
TCTAACG42000.027.4166665
CTCACCC136650.026.1741662
CTAACGC44300.025.9932276
GAGTCTA53650.021.8136062
CCTCACC171200.021.4684561
GTAGCAT104850.021.0233657
TAGCATA106600.020.4136968
GTCTAAC58950.019.8524174
CGGACAC126400.019.39121612-13
GCCAGTA117150.018.8561653
CCAGTAG117750.018.6802524
GACACGG133000.018.46428514-15
GTCGAAC25650.018.3235861
Produced by FastQC (version 0.11.5)