FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_75_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_75_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19267956
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT301190.15631652885235986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG50800.036.9033667
CGCTCGA9100.036.7625661
AGACGCG22250.035.7987564
AACGCGC53250.034.86598
TCACCCG107100.034.3475843
TCTAACG55050.034.14215
CTAACGC56900.033.1169476
CTCACCC120150.031.0145382
GAGTCTA69850.027.8716262
CCTCACC139700.026.9736141
GTCTAAC73700.025.912274
AGTCTAA79900.024.1135753
CGCAGGC58050.021.8825683
GCCGCAG62000.021.5082421
CACCCGG179150.020.4896494
CGGACAC124850.019.746412-13
TCAGACG45100.018.9564062
GCGGTGT85150.018.334511
GACACGG137150.018.02866214-15
GCCCGGA118150.017.53144310-11
Produced by FastQC (version 0.11.5)