FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_74_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_74_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21346263
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT288470.13513840806702326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG85550.032.13913
TAACGCG29500.031.7050867
AACGCGC31600.029.7468388
CGCAGGC78000.029.7064133
CGCTCGA12450.029.0682751
GCCGCAG86000.027.4348831
CTAACGC38050.024.7043386
TCTAACG39350.024.0076245
CTCACCC141150.019.745662
CGGACAC109100.017.14573912-13
TCGTGCG30500.016.950827
CCTCACC168300.016.9233531
GCGGTGT75100.016.8348871
GACACGG114200.016.40061414-15
CCGCAGG138650.016.372882
GAGTCTA62250.015.8554222
CACCCGG175350.015.8140874
GTCGAAC34750.015.6892091
CGGTGTG76950.015.4528922
TTCCCGG148950.015.43001194
Produced by FastQC (version 0.11.5)