FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_74_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_74_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21346263
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT230830.10813602362155848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG39550.034.095227
AACGCGC39800.033.00288
TCACCCG90550.031.1909283
CTAACGC47200.028.7739936
TCTAACG48450.028.1313845
CGCTCGA11150.027.0848051
AGACGCG20100.025.7885634
CTCACCC121700.023.554932
CCTCACC140200.020.8112831
CACCCGG141050.020.0420274
GTCTAAC69450.019.972164
GAGTCTA74900.018.9813392
GTCGAAC29600.018.0506731
CGGACAC110600.017.3452112-13
AGTCTAA83650.016.7689573
GCGGTGT79100.016.4463211
CGCAGGC62100.016.4277363
GCCGCAG68700.016.095611
GACACGG122750.015.89408914-15
GTCGGTA20600.015.5621321
Produced by FastQC (version 0.11.5)