FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_70_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_70_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55130008
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2773050.5030019222924836No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC2485630.4508669761121747No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT2328470.4223598153658893No Hit
CGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC2155580.39099939909313997No Hit
GCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1859000.33720292585482664No Hit
CTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1789260.3245528279263083No Hit
GCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1524520.27653179371931164No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA1503820.27277703279128857No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT1496150.27138577596433505No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA1427930.259011389949372No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC1315990.23870665863135734No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA1219460.2211971382264265No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA1147020.20805728887251387No Hit
GTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAACGGC1048570.19019950078730263No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC1041710.18895516938796744No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG1030750.18696714137970014No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAAC1027480.18637399798672258No Hit
GCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTC936340.16984216653841228No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACT888310.16113003284889782No Hit
GCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCT883280.16021764408232989No Hit
CCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAA876350.1589606154238178No Hit
CGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGA848930.15398691761481334No Hit
GTTCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATT844100.15311080673160796No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC827290.1500616506349863No Hit
GGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT792680.14378376291909845No Hit
CCCTTCTCCACTTCAGTCCTCCAAGGTCTCATTCGATTATTTGCTACTAC751940.13639395807814864No Hit
CTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACA741900.13457280833334906No Hit
CACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGT715160.1297224553277772No Hit
ATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTA690910.125323761970069No Hit
GCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTG641430.11634861362617614No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT637590.11565207826561535No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACAC623680.11312895147775055No Hit
CCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTT615940.11172499739162019No Hit
CATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTT610750.11078358631836221No Hit
CCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA596200.1081443703037373No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGA580300.10526027857641522No Hit
CCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTG576460.10456374321585443No Hit
GTAAAATGCTTATTGTGTTTGAATGTGTTTATGTAAGTGGAGCCGTACCT554380.10055866489262981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT610300.042.5333441
TTCGTAC250350.039.6876376
CCTTTCG276550.035.961673
GCGCGTG806300.034.3158872
GTCCTTT304500.033.5559961
TTTCGTA304350.033.0783655
CGCGTGC848150.032.65593
CGCAGGC461550.031.8323043
GCGTGCA886700.031.4693814
GGGCTTA194800.031.1724854
TCCTTTC321650.031.1238922
GCGGTGT812000.030.3184721
CGTGCAG933400.029.995615
CGGTGTG843550.028.7666422
GCAGCCC972650.028.707868
CCGCAGG506650.028.2194822
CAGCCCA1000700.027.9830119
TAACCTC214050.027.9738399
GGTGTGT933700.027.0462673
TGCAGCC1056200.026.5125927
Produced by FastQC (version 0.11.5)