FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_70_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_70_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55130008
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC2449600.44433151542441274No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT2092070.37947935723136483No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1847290.3350788557839498No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA1474870.2675258091745606No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC1230920.22327586094310017No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA1004520.18220929697670277No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG980660.17788134549155155No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACT877010.1590803324389142No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC875280.15876652874782823No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC858640.15574820885206475No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA747580.13560310022084524No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA730770.1325539441242236No Hit
CGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC695490.12615452549907122No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT661760.12003626046997852No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT614520.11146742441974615No Hit
GACAGATTGATAGCTCTTTCTCGAATCTATGGGTGGTGGTGCATGGCCGT611940.11099943972436935No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACAC605710.10986938365762618No Hit
GGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT593480.10765099109000674No Hit
CCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAA590550.10711952009874551No Hit
GCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC589920.1070052447661535No Hit
GCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCT589710.1069671529886228No Hit
GTTCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATT584140.10595681393697602No Hit
AAACTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTT566830.10281696313194802No Hit
CACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGT563360.10218754185560794No Hit
GCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTC562300.10199526907378646No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA553890.10046978407839158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC271650.044.921326
CCTTTCG283850.042.9702643
CGCGCGT520700.042.744091
GTCCTTT312600.040.700091
TCCTTTC319100.039.1572342
TTTCGTA328550.037.332315
GCGCGTG698800.033.9255452
CGCGTGC708250.033.2518233
CATATGG121350.032.2352453
GCGTGCA748950.031.7830244
GGGACTA122950.031.4767638
TCGTACT393350.031.3660167
CGTGCAG794500.029.8145435
GCGGTGT1019100.029.781011
GCAGCCC798450.029.3662418
CGGTGTG1051050.028.5174852
CAGCCCA827850.028.343559
TAACCTC161150.027.203299
GGTGTGT1148150.027.1574843
GGGCTTA166450.027.07294
Produced by FastQC (version 0.11.5)