FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_69_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_69_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40935447
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT2106190.51451496303436No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1838350.44908511686705166No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA1427030.34860496332188584No Hit
CGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1376540.33627090965929846No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC1347780.3292452138118829No Hit
CTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1152420.28152129375795015No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT1053720.2574101609297194No Hit
GCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1024070.25016704959884767No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC980070.23941841895607002No Hit
GCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG928120.22672770618579052No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC849280.20746811437041351No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA837190.20451468381425028No Hit
CCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAA822900.20102382172594818No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG788170.19253973212995573No Hit
GCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTC728630.1779948805737971No Hit
GTTCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATT708060.17296989574829852No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA685860.1675467230148971No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA640950.156575791147462No Hit
CTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACA634910.1551002973046807No Hit
GTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAACGGC604390.14764465623155404No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACT591090.14439563833271443No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAAC586740.1433329896214398No Hit
CATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTT562630.1374432286033178No Hit
GCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTG553340.13517380181533134No Hit
GGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT542180.13244755822502682No Hit
ATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTA537740.13136292367834654No Hit
CCCTTCTCCACTTCAGTCCTCCAAGGTCTCATTCGATTATTTGCTACTAC537200.13123100866591245No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT520110.1270561428094336No Hit
CACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGT513560.1254560625660201No Hit
CCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA501820.12258813248087898No Hit
CGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGA487850.11917544225179709No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC485860.11868931100227145No Hit
GCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCT481870.11771460563261957No Hit
CCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTT437350.10683894571860912No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA436080.10652870115232893No Hit
GTAAAATGCTTATTGTGTTTGAATGTGTTTATGTAAGTGGAGCCGTACCT433560.10591309776096984No Hit
CCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTG423130.10336518372451142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT408150.043.96571
TTCGTAC188300.041.2092446
CCTTTCG215050.036.34553
GCGCGTG531700.035.9416962
GTCCTTT232950.034.420261
CGCGTGC556550.034.260893
GCGTGCA585650.033.040044
TTTCGTA237800.032.9671975
TCCTTTC247900.032.116982
CGTGCAG625600.030.9601975
GCAGCCC651450.029.7172458
CGCAGGC344000.029.6345923
GGGCTTA134950.029.0114864
CAGCCCA676350.028.7274349
GGGACTA63400.027.5772868
TGCAGCC714950.027.301357
CCGCAGG366000.026.68472
TAACCTC146650.026.664859
GCGGTGT512050.026.646031
TCGTACT304500.026.1471277
Produced by FastQC (version 0.11.5)