FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_69_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_69_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40935447
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT1449950.3542040227385327No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC1425420.34821166115518415No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1337680.3267779145052453No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA902110.22037379975354854No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA878030.21449136734722846No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG755000.18443673034766178No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC739140.18056233757506057No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACT633740.15481448144440685No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC631740.1543259073242806No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA575550.14059941741933343No Hit
GTTCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATT572650.13989098494515034No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT567610.13865977816243216No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC535880.13090854974662913No Hit
CCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAA512750.125258190047369No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA489550.11959073025390439No Hit
GCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTC470450.11492484740669864No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT459270.11219371807519288No Hit
GTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAACGGC445770.10889584276434065No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACAC421990.10308669647603945No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA417030.10187503265812635No Hit
CATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTT416110.10165028856286827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC197650.045.2831046
CCTTTCG206500.043.5291863
CGCGCGT378900.042.512851
GTCCTTT224150.041.1445921
TCCTTTC230000.039.714282
TTTCGTA238500.037.8107455
GCGCGTG505450.033.9922372
CGCGTGC515750.033.373443
GCGTGCA545800.032.0789764
TCGTACT289700.031.1783267
CGTGCAG577150.030.1527125
GCAGCCC583500.029.7770238
CATATGG90300.029.2405723
CAGCCCA601550.028.9142279
GGGACTA87850.028.888458
CCTGCCA168000.028.3745561
CGTACTA317100.027.7780328
TGCAGCC652550.026.9723117
GCGGTGT650150.026.7698061
CGGTGTG676350.025.5473382
Produced by FastQC (version 0.11.5)