FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_68_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_68_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62350998
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC3283210.526568957244277No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC3235720.5189523991259931No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT2908810.4665218029068276No Hit
CGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC2480300.39779635924993534No Hit
CTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA2094870.33598018751840986No Hit
GCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC2015450.32324262075163573No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA1873440.3004667222808527No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA1816460.29132813559776544No Hit
GCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1812160.2906384914640821No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT1751300.2808776212371132No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC1650880.2647720249802577No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC1154290.18512775048123528No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA1152140.18478292841439364No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG1146400.18386233368710472No Hit
GCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTC1078880.17303331696471No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC1050510.16848326950596684No Hit
CCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAA1033790.16580167650243546No Hit
CGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGA1009080.16183862846910646No Hit
GTTCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATT989930.15876730633886565No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA974190.1562428880448714No Hit
CCCTTCTCCACTTCAGTCCTCCAAGGTCTCATTCGATTATTTGCTACTAC973050.15606005215826696No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACT955900.15330949474136726No Hit
GCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCT933590.14973136436404755No Hit
CTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACA874860.14031210855678686No Hit
GCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTG849050.13617263993112028No Hit
CACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGT814980.13070841303935504No Hit
CATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTT790450.12677423383022673No Hit
ATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTA770630.12359545552101667No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAAC759030.12173502018363845No Hit
GTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAACGGC756020.12125226929006012No Hit
GGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT733700.1176725350891737No Hit
CCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTT726430.11650655535617889No Hit
GTAAAATGCTTATTGTGTTTGAATGTGTTTATGTAAGTGGAGCCGTACCT707360.11344806381447174No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA703180.1127776655635889No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACAC695490.11154432524079247No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT685920.1100094660874554No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGA681730.10933746401300586No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC673310.10798704456983993No Hit
CCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTG643670.10323331151812518No Hit
GTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAAT639550.10257253620864257No Hit
CCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA630280.10108579176230667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT674000.044.9149861
TTCGTAC292600.038.8079266
GCGCGTG880200.036.4540982
CCTTTCG321050.035.3543053
CGCGTGC926300.034.7261163
GCGTGCA969850.033.4090844
GTCCTTT346850.033.252991
CGCAGGC546400.032.3770143
GGGCTTA213900.031.8606824
TTTCGTA358800.031.85735
GCGGTGT1010500.031.604651
TCCTTTC365050.031.5693742
CGTGCAG1029150.031.511445
GCAGCCC1070250.030.296948
CGGTGTG1046050.030.2070662
CCGCAGG586500.029.4421142
CAGCCCA1103250.029.4418329
GGTGTGT1154950.028.4006233
TAACCTC240550.028.1941389
TGCAGCC1166800.027.934957
Produced by FastQC (version 0.11.5)