FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_68_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_68_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62350998
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC3288690.5274478525588315No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2156210.34581804127658067No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT2131950.34192716530375344No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA1932030.30986352455817945No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC1209530.1939872718637158No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG1189580.19078764384813857No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA1159490.18596173873592206No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACT1040260.16683935035009384No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC1023630.1641721917586628No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC920700.1476640357865643No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT877350.1407114606248965No Hit
GTTCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATT844270.1354060122662351No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA841170.13490882696055642No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACAC799160.1281711641568271No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT780410.12516399496925454No Hit
CGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC744900.11946881748388374No Hit
GCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTC734840.11785537097577814No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA712090.11420667236152339No Hit
GCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCT701950.11258039526488414No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA700080.11228048025791022No Hit
GTAAAATGCTTATTGTGTTTGAATGTGTTTATGTAAGTGGAGCCGTACCT698370.1120062264280036No Hit
GCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC684160.10972719313971527No Hit
CCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAA623750.10003849497324806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC306350.045.128296
CCTTTCG323100.043.3984683
CGCGCGT586550.042.90651
GTCCTTT352400.041.066861
TCCTTTC362600.039.2838172
TTTCGTA365850.038.106075
GCGCGTG791700.034.0123832
CGCGTGC799850.033.4609953
GCGTGCA844550.031.7172644
TCGTACT449350.031.4232237
GCGGTGT1278100.031.191941
CGGTGTG1319450.029.8728772
CGTGCAG895250.029.7456445
GCAGCCC897750.029.4481018
CAGCCCA928600.028.4473329
GGTGTGT1440400.028.1680223
GGGCTTA172100.027.6804624
AATCCCA1457750.027.21342794
CGTACTA512800.026.9518898
TAACCTC168850.026.7556889
Produced by FastQC (version 0.11.5)