FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_67_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_67_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47168362
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA1252980.26563992194598574No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT1172570.24859247815304675No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC1055810.22383859757521365No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA975240.20675723274003027No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC966370.2048767349606077No Hit
GTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAACGGC891240.1889486855617331No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT847390.17965219992163392No Hit
CCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA834990.17702331914769479No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG824120.17471880834021752No Hit
CGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC741120.1571222676759477No Hit
AAACTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTT667140.14143802576820455No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA616220.13064265407393202No Hit
CTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA609930.1293091331007No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC586570.12435666093302117No Hit
GCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTG575900.12209455142834935No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC572400.12135252862925366No Hit
GCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTC557750.11824663319875302No Hit
CTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCAT548930.11637673574503181No Hit
GCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG538810.11423122982307506No Hit
CTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG529250.11220444754897362No Hit
GGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT518940.11001866038935165No Hit
CACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGT496140.10518491186952814No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT495930.1051403905015824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC225950.034.488166
GTCCTTT242300.033.2278181
CCTGCCA249450.032.0116231
CCTTTCG251100.031.277183
TTTCGTA258200.030.1986835
TCCTTTC281350.028.2651482
ACTTACC235850.026.6834853
CGTACTA288200.026.6474678
GTAGTTA313150.026.6105698
GCCAGTA314900.026.1194044
CCAGTAG324900.025.4890755
CGCAGGC363650.023.7035623
CAGTAGT351250.023.6438456
CTTACCA421900.023.3607481
AGTAGTT366350.023.1953037
TAGTTAT374450.022.5052749
TCTTACA358150.022.36158294
TCGTACT365450.021.6319627
CGCGCGT426900.021.5898341
TTACCAG462350.021.3677962
Produced by FastQC (version 0.11.5)