FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_67_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_67_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47168362
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC994740.21089135976356355No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT810210.17176979773009712No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG757970.1606945774373085No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA755910.16025784401841217No Hit
AAACTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTT699090.1482116338913783No Hit
CCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA669460.14192988088074798No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA666500.14130234159922703No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA653610.13856957763341454No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC641620.13602761953022666No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC628290.13320157269824212No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT558150.11833143580436395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC225000.040.2888876
GTCCTTT237650.039.3715551
CCTTTCG243900.037.4883233
TTTCGTA248050.036.5645525
TCCTTTC260950.035.4644242
CCTGCCA237150.032.911451
CGTACTA300200.029.854038
ACTTACC285000.028.7329713
TCGTACT341700.026.9396537
GTAGTTA299050.026.5343938
GCCAGTA320150.025.0587734
CCAGTAG319000.024.898935
CTTACCA488400.024.3800071
CAGTAGT344450.023.1013816
AGTAGTT355250.022.8620327
TTACCAG525350.022.7802262
TAGTTAT357650.022.6165359
GTACTAA403050.022.1433629
GTCTCGT377500.020.8107411
TACCAGG572800.020.6565693
Produced by FastQC (version 0.11.5)