FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_66_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_66_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53849550
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC2280680.42352814461773586No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT2165080.4020609271572372No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2060220.38258815533277435No Hit
CGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1919230.3564059495390398No Hit
GCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1715610.3185931915865592No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA1615490.30000064995900616No Hit
CTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1508970.2802196118630518No Hit
GCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1388220.25779602615063635No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA1376520.25562330604434025No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT1295620.24059996787345483No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC1094850.20331646225455924No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG924150.17161703301141792No Hit
CCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAA909640.16892248867446433No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA883690.16410350690024336No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC855750.15891497700537888No Hit
CGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGA763590.14180062786039996No Hit
CTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACA729100.135395746111156No Hit
GCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTC711270.1320846692312192No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACT708920.1316482681842281No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC705790.13106701913015056No Hit
GCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCT702570.13046905684448615No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA652130.1211022190528983No Hit
GTTCTTATATGTGATTAAATACTTGTATTTTTTCATATGTTCCTCCTATT601130.11163138782032532No Hit
CATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTT589630.10949580822866671No Hit
CCCTTCTCCACTTCAGTCCTCCAAGGTCTCATTCGATTATTTGCTACTAC554960.10305750001624897No Hit
GGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT554420.10295722062672762No Hit
GTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAAT543530.1009349196047135No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAAC540070.10029238870148405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT473550.041.5461961
TTCGTAC251200.035.0067676
GCGCGTG648450.032.304572
GTCCTTT281000.031.6790031
CGCGTGC691250.030.4607583
GCGTGCA706700.029.9278324
CGCAGGC450200.029.6908023
CCTTTCG299500.029.4240383
GCGGTGT800450.028.9826981
TTTCGTA304800.028.9740815
TCCTTTC326200.027.3614372
CCGCAGG477350.027.2734892
CGTGCAG778950.027.2243395
CGGTGTG828150.027.1619872
GGGCTTA159350.027.0467534
GGTGTGT896500.026.0033453
GCAGCCC836800.025.319798
CGTACTA346700.025.0928758
GAGCACT877700.024.22558894
GCGCGCG289300.023.7030751
Produced by FastQC (version 0.11.5)