FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_65_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_65_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46275516
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1561470.3374289764807809No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT1477260.31923144844025075No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA1318720.28497143068053526No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC1278310.27623895106863855No Hit
CGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1218610.2633379604022136No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT1049540.22680244127369645No Hit
GCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1037730.2242503357499028No Hit
CTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA997410.21553730486765396No Hit
GCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG879620.19008323969850494No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC874150.18890118912990617No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG799610.17279331904154238No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA798240.1724972661569025No Hit
CCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAA678060.14652672916710427No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA664080.14350569316180073No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC623990.134842364588652No Hit
GCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTC607150.13120329117453816No Hit
CTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACA596370.1288737655567147No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA554300.11978256493131272No Hit
GCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCT537070.11605921368872472No Hit
CCCTTCTCCACTTCAGTCCTCCAAGGTCTCATTCGATTATTTGCTACTAC535990.11582582893295021No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC508200.109820493411678No Hit
CGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGA474980.10264175120165057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT386750.039.2527471
TTCGTAC226450.034.5572976
GTCCTTT243500.033.0833661
GCGCGTG530500.030.3440132
CCTTTCG266200.029.6619073
TTTCGTA268000.029.5854475
CGCGTGC555150.029.123663
GGGCTTA158350.029.0281014
GCGTGCA578100.028.3089434
TCCTTTC288650.027.6317332
CGCAGGC394500.026.7703423
CGTGCAG641400.025.551765
CCGCAGG407650.025.3879552
CGTACTA320050.024.230598
TAACCTC201200.024.0606359
GCAGCCC679350.024.0067718
GCGGTGT548700.023.427191
CCCCAAA565150.022.6953891
TGCAGCC744350.022.251367
GTACTAA347850.022.199519
Produced by FastQC (version 0.11.5)