FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_65_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_65_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46275516
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC1415050.30578805431364614No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1054480.22786996043436877No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT940420.2032219370606262No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG897490.19394489301858894No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA867760.18752032932490692No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA744720.16093175492629838No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC589350.1273567646441803No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC573120.12384951039767983No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACT518900.11213273126981448No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA484980.10480272116252577No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA483630.10451099021780762No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC481740.10410256689520221No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT472890.10219010847982765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC246050.042.7146076
GTCCTTT257750.042.2273181
CCTTTCG272150.039.0802733
TTTCGTA280950.037.666885
CGCGCGT330700.037.6320951
TCCTTTC289250.037.5323072
TCGTACT362900.029.5609687
CGTACTA356100.029.314168
GTACTAA366100.028.4346949
CGCGTGC473600.028.0963613
GCGCGTG482000.027.8432792
GCGTGCA517700.025.825744
CATATGG108300.025.2716433
GCGGTGT701950.025.1410271
CGTGCAG568800.023.573115
GCAGCCC568550.023.3195468
CGGTGTG735250.023.3073832
GGTGTGT780500.022.941433
ACTTACC165750.022.7864483
GGGCTTA132500.022.669294
Produced by FastQC (version 0.11.5)