FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_73_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_73_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21074761
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGATCGTCCTTCAAAATTATCTGAGCTTTTTCACTCAGAAATGAAAT882710.41884698004404414No Hit
GCTCGAATGGCTTTTAACCCCATGTCTTTATTTTTAAAAATTATGCCACA840360.39875185298661275No Hit
GCCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAAATTCAAATT499420.23697540389663257No Hit
GCCACATCTAGTAAACTAAAAACATTACTCGCCTAATTTCGGGATTTATT420800.19967011725542227No Hit
GCCATTATTAAACCTGAAATCATTCCGCTACATTTTTCTATTATACCACA366640.17397113068091258No Hit
CGCTGTTATCCCTAGGGTAACTTAGTCTTGTAATCTCACTCTAAGGATCA341300.16194726953249908No Hit
GTTATATTTTACTCCCTGTCGCCCCAACAAAATTTACTTTCCATTCAATC339280.1609887770494764No Hit
GGCCATTATTAAACCTGAAATCATTCCGCTACATTTTTCTATTATACCAC336150.1595035882020204No Hit
GCCGGTTTGAACTCAGATCATGTAAGAGATTAAAAGTCGAACAGACTTTC324480.15396615885703283No Hit
CTCCCTGTCGCCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAA286030.13572158659355615No Hit
GTCTCAATTTCCTAATCCTTTCGTACTAAAGAAACTACCATAATTTTGAG285760.13559347126166696No Hit
CCCCAATTAAAAGACTAATGATTATGCTACCTTAGCACAGTCAGAATACT236050.11200601515718256No Hit
CTGCACCTTGCCAATCTCTTAATCCAACATCGAGGTCGCAAACCTTTTTA230390.10932033819980212No Hit
CTCGAATGGCTTTTAACCCCATGTCTTTATTTTTAAAAATTATGCCACAT228800.10856588124534365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAA222100.035.742011
TCGAATG224150.035.4360923
CTCGAAT228050.034.6033742
CATACCA282100.030.3059294
ATGGCTT284150.028.5820877
CGAATGG285350.028.4618874
AATGGCT311000.026.356276
GAATGGC320650.025.4604685
TAACCCC234450.021.75090614-15
GGCTTTT373750.021.7174579
ACCCCAT236100.021.67852416-17
CCTCACC76150.021.2934971
TAAACCT157250.021.1313199
TTAACCC238850.020.95666714-15
AACCCCA242750.020.74582916-17
CCCCATG244950.020.59787618-19
GCCATTA166450.020.4433752
TGGCTTT402050.020.3524448
CCCATGT254250.020.07551418-19
CCATTAT171650.019.824063
Produced by FastQC (version 0.11.5)