FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_73_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_73_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21074761
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGATCGTCCTTCAAAATTATCTGAGCTTTTTCACTCAGAAATGAAAT476230.22597172039104024No Hit
GCTCGAATGGCTTTTAACCCCATGTCTTTATTTTTAAAAATTATGCCACA427740.20296315578620322No Hit
GCCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAAATTCAAATT256190.12156246991365643No Hit
GTATGAATGACGAGACGAGAAAATGACTGTCTCTATAATAAACTTTGAAT255240.12111169374589824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT168100.030.0476632
TCGAATG172500.029.2021163
GCTCGAA176950.028.8862381
CATACCA209750.027.58403494
ATGGCTT210550.024.086567
CCTCACC68550.023.9963231
TTAACCC153250.021.19017214-15
CGAATGG249650.020.8428544
CCCCATG162450.020.60199418-19
GGCCCGG75750.020.4044599
GAATGGC255650.020.1207665
AACCCCA167250.020.04357316-17
TAACCCC156800.019.95926714-15
ACCCCAT157500.019.74791516-17
AATGGCT265850.019.7121416
CCCATGT165700.018.96795818-19
CCATTAT129000.018.6646353
TTAAACC127400.018.6131889
GCCATTA130500.018.1596932
CTCACCA92750.017.8855862
Produced by FastQC (version 0.11.5)