FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_72_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_72_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28492134
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCAGGCCCGGACACTGGAAGGATTGACAGATTGAGAGCTCTTTCT445520.15636596402361438No Hit
GTCTGATCGTCCTTCAAAATTATCTGAGCTTTTTCACTCAGAAATGAAAT426800.1497957295862781No Hit
GCCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAAATTCAAATT387260.13591821518177613No Hit
GCTCGAATGGCTTTTAACCCCATGTCTTTATTTTTAAAAATTATGCCACA332820.11681118725610375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCACC134100.040.6888051
CTCACCA183800.030.4197562
GGCCCGG196950.027.724689
CCAGGCC210750.026.127786
TCACCAG212350.025.827343
CAGGCCC225750.024.6480187
CTCGAAT162550.024.074692
AGGGGAT111650.023.9251697
CATACCA207200.023.91710794
CACCAGG228350.023.8767094
TCGAATG167000.023.6070673
CCCGGAC162950.023.00881410-11
ACCAGGC236550.022.8092565
CTGCCAG113450.021.3436571
AGGCCCG286500.019.4604648
ACCCCAT141400.019.26651416-17
AAGGGGA144000.018.9521266
TCCGGTC101650.018.56325512-13
ATGGCTT220550.018.495557
GACACTG211800.018.02860514-15
Produced by FastQC (version 0.11.5)