FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_71_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_71_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19212143
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGAATGGCTTTTAACCCCATGTCTTTATTTTTAAAAATTATGCCACA790340.41137524325110425No Hit
GTCTGATCGTCCTTCAAAATTATCTGAGCTTTTTCACTCAGAAATGAAAT636470.33128527098720845No Hit
GCCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAAATTCAAATT584270.30411495479707806No Hit
GCCACATCTAGTAAACTAAAAACATTACTCGCCTAATTTCGGGATTTATT425350.2213964366182367No Hit
GCCGGTTTGAACTCAGATCATGTAAGAGATTAAAAGTCGAACAGACTTTC379220.19738558056745675No Hit
GCCATTATTAAACCTGAAATCATTCCGCTACATTTTTCTATTATACCACA314420.16365691219350179No Hit
CCCCAATTAAAAGACTAATGATTATGCTACCTTAGCACAGTCAGAATACT299020.15564114841327176No Hit
CGCTGTTATCCCTAGGGTAACTTAGTCTTGTAATCTCACTCTAAGGATCA297730.1549696980706421No Hit
GGCCATTATTAAACCTGAAATCATTCCGCTACATTTTTCTATTATACCAC282290.14693311412474913No Hit
CTCCCTGTCGCCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAA255040.13274937626687455No Hit
GTTATATTTTACTCCCTGTCGCCCCAACAAAATTTACTTTCCATTCAATC248440.12931404893249027No Hit
GTCTCAATTTCCTAATCCTTTCGTACTAAAGAAACTACCATAATTTTGAG236740.12322415047608172No Hit
CTGCACCTTGCCAATCTCTTAATCCAACATCGAGGTCGCAAACCTTTTTA219790.11440160527641295No Hit
CACCTTGCCAATCTCTTAATCCAACATCGAGGTCGCAAACCTTTTTATCG214020.11139829637953455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAA191450.039.7947241
TCGAATG206550.036.5214233
CTCGAAT207150.036.279512
CGAATGG237000.032.26544
GAATGGC266400.028.969225
ATGGCTT264450.028.9340117
AATGGCT284250.027.2327186
GGCTTTT298650.025.7150529
CATACCA242850.023.30162694
TAACCCC201000.023.13756214-15
TGGCTTT338500.022.9098978
TTAACCC207050.022.7225314-15
TAAACCT146400.022.5689899
GCGCTGT72550.022.2205371
ACCCCAT213700.021.91647116-17
CCCCATG217400.021.7272318-19
AACCCCA227600.020.83611716-17
GCCATTA162200.020.6023431
CCATTAT159000.020.4257852
GCTTTTA241800.019.7874310-11
Produced by FastQC (version 0.11.5)