FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_71_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_71_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19212143
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGATCGTCCTTCAAAATTATCTGAGCTTTTTCACTCAGAAATGAAAT433930.2258623621529363No Hit
GCTCGAATGGCTTTTAACCCCATGTCTTTATTTTTAAAAATTATGCCACA399870.208133991090947No Hit
GCCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAAATTCAAATT314220.16355281136518712No Hit
GTATGAATGACGAGACGAGAAAATGACTGTCTCTATAATAAACTTTGAAT246910.12851767759588298No Hit
GCCACATCTAGTAAACTAAAAACATTACTCGCCTAATTTCGGGATTTATT222140.11562479000911038No Hit
CCCCAATTAAAAGACTAATGATTATGCTACCTTAGCACAGTCAGAATACT203440.1058913625616882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAA157650.031.7484951
CTCGAAT159900.030.4879652
TCGAATG161450.030.331763
CGAATGG195050.025.7174134
CATACCA201600.024.14469394
ATGGCTT206350.023.8293537
GAATGGC220900.022.5656135
TTAACCC133900.022.19929114-15
CCCCATG140650.021.5251818-19
AATGGCT234750.021.4398256
GGCTTTT224750.021.4360799
TAACCCC135500.021.08523214-15
CTAATAC73700.020.6246037
ACCCCAT147650.019.33938216-17
TGGCTTT253450.019.2686778
AACCCCA158950.019.21681216-17
GCCAGGC88000.018.694851
TTTAACC158100.018.12476212-13
GCTTTTA169650.017.93967610-11
CCCATGT157700.017.86000618-19
Produced by FastQC (version 0.11.5)