FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_79_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_79_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24787128
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT633560.2556004067917832No Hit
CCTCGATCTATTAGTATTCGTCAGCTCCACATGTCACCATGCTTCCACCT387950.15651268674612082No Hit
CTCACCCGGTCCGGACACCATTAGGACTGACAGATTGAAAGCTCTTTCTC297570.1200502131590235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA26900.046.4758341
GCCGCGA19600.039.5663261
TCTATTA104500.038.049767
CGATCTA103800.037.763014
GTCCTCG67150.037.1660461
ACGCTAT112650.033.41943794
CTATTAG122450.032.2033468
CCCCTGT14450.031.5501731
GCCGCAA196500.031.070231
GAGTACC68950.030.8107326
TATTAGT131800.030.1327769
TCGATCT134850.028.9981483
GCCCCTC10900.028.4587151
GCTAGGC7550.027.3907281
ATCTATT149450.026.7942476
GGTGCCC158450.026.5774722-23
GATCTAT152700.026.2239695
GGGGCGT8900.025.8764041
TAGGCGT10250.025.678051
GCCGCGC20950.025.575181
Produced by FastQC (version 0.11.5)