FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_79_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_79_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24787128
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT318450.12847394018379216No Hit
GTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAA254510.10267829334644982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT126450.041.065464
GCCCGGA26900.040.9363331
CTGGCTC131100.039.1045729
GTTTGAT195050.038.471531
CCTGGCT140050.036.7422838
GCACGGT74550.031.0933674
GTCCTCG55000.030.9194151
TCTATTA62750.030.7285757
CCCCTGT10350.030.5268121
ACGCTAT60650.030.42251894
TCCTGGC173550.030.045547
GCCGCGA17800.029.757591
GTCTGTT91200.027.7660771
GGTGCCC90400.027.19022422-23
GGCGCAC88550.027.0753461
GCCCCTC7900.026.4666081
GATCATG64650.025.6394585
GGCTCAG134450.025.60687410-11
CTAATAA97350.025.3131458
GGGCGCA63200.024.1140211
Produced by FastQC (version 0.11.5)