FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_78_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_78_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20924138
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT692930.3311629850653824No Hit
CTCACCCGGTCCGGACACCATTAGGACTGACAGATTGAAAGCTCTTTCTC268320.12823467327542956No Hit
CCTCGATCTATTAGTATTCGTCAGCTCCACATGTCACCATGCTTCCACCT267410.12779976886025127No Hit
CCCGGACATGGAAGAATTTCAACGGTTTACCGATACCTTTCGGCATAGGA247380.11822709255693113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA25800.047.5465131
GCCGCAA189600.037.109181
GCCGCGA20300.036.3497541
CGATCTA75200.036.04
TCTATTA78200.034.7391327
GCTAGGC7250.031.7655181
ACGCTAT81800.030.96943994
GTCCTCG59800.028.8444821
GGTGCCC177300.026.62803322-23
CGGTCCG97900.026.548527
CTATTAG102750.026.5304158
GATCTAT105300.026.4235525
TAGGCGT9300.026.279571
CCCCTGT15100.026.1456951
GAGTACC50550.026.1266086
CGCAAGC273050.026.008793
ATCTATT107700.025.6601666
GTGCCCT200650.025.2275122-23
GGGGCGT9250.024.3891891
TCGATCT112900.024.353413
Produced by FastQC (version 0.11.5)