FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_78_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_78_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20924138
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT344360.16457547737450404No Hit
CTCACCCGGTCCGGACACCATTAGGACTGACAGATTGAAAGCTCTTTCTC209930.10032910316305503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA28750.044.284561
GTTTGAT141400.030.7257041
GCCGCGA20000.028.3445451
TGATCCT112050.027.651894
CCTGGCT107950.027.4928368
GGTGCCC101500.027.49124122-23
CTGGCTC109700.026.7648859
GCCGCAA165800.025.9517151
ACGCTAT41750.025.7608594
TCTATTA45050.024.8753557
GCACGGT51400.024.634574
GAAACGT82900.024.3555455
GGCGCAC58100.023.3531971
TCCTGGC131750.022.6942677
AAACGTC90550.022.5646216
GTGCCCT129250.022.46964522-23
CACGCCT126900.022.1783812-13
GTCTGTT104800.021.9474241
GGCTCAG90800.021.88039210-11
CTAATAA102700.021.829978
Produced by FastQC (version 0.11.5)