FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_77_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_77_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26398745
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT1073930.4068110055989404No Hit
CTCACCCGGTCCGGACACCATTAGGACTGACAGATTGAAAGCTCTTTCTC423980.1606061197227368No Hit
GTCTGTTCTAATAATTGCACCCCGCCTTGCGTTGGGGTATAGTTGCATGT386300.14633271392257471No Hit
GCATCGTTTACGGTCAGAACTAGGGCGGTATCTAATCGCCTTCGAACCTC317490.12026708087827662No Hit
CCCGGACATGGAAGAATTTCAACGGTTTACCGATACCTTTCGGCATAGGA279620.10592170195969544No Hit
CCGCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC277720.10520197077550465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA33550.046.929961
GCCGCAA248800.042.428461
GCCGCGA29350.040.0340731
GCTAGGC10750.036.7255821
CGGTCCG141000.030.07
CGATCTA65750.028.7361224
GCCCGGT41700.028.0647491
CGCAAGC387950.027.634233
GCCGCGC22600.027.4513261
GGTGCCC273250.026.70356822-23
GTGCCCT292050.025.80534222-23
GGTCCGG166750.025.4236878
ACGCTAT73400.025.1648594
CACGCCT292600.024.97778512-13
TCTATTA81750.023.859337
CCCGGTC181350.023.4546455
GTCTGTT173950.023.344641
CCCCTGT19800.023.2626291
GCAAGCT490950.021.9706694
CTCCACG552300.021.929939
Produced by FastQC (version 0.11.5)