FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_77_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_77_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26398745
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT520140.19703209376051778No Hit
CTCACCCGGTCCGGACACCATTAGGACTGACAGATTGAAAGCTCTTTCTC374020.14168097763738388No Hit
GTCTGTTCTAATAATTGCACCCCGCCTTGCGTTGGGGTATAGTTGCATGT322780.12227096401741826No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTGCTAAA282420.10698235844166076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGA36250.038.5934831
GCCGCAA199300.033.5823171
GCCGCGA24100.030.8573341
GCTAGGC9650.029.380421
GGTGCCC155650.028.1265822-23
CACGCCT162450.026.43298712-13
GTTTGAT147150.025.9953141
GTCTGTT159550.025.4606881
GTGCCCT178450.025.35590422-23
GGCGCAC55300.023.7016981
CGCAAGC283600.023.593963
CTAATAA164700.023.4742648
CGGTCCG184700.022.7672587
TCTAATA172450.022.678737
GGGCGCA43400.022.2755811
TCTGTTC180700.021.9662132
CTCCACG334850.021.9307049
GCTCCAC340750.021.7939418
CGCACGG57550.021.7132723
CTGTTCT183850.021.5190473
Produced by FastQC (version 0.11.5)