FastQCFastQC Report
Thu 20 Oct 2022
X3-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameX3-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16315347
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA425610.26086481642100534No Hit
CGACATGGCTACGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212150.13003094571019544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG4238550.086.580551
GACATGG4359100.084.4018252
ACATGGC4366550.084.272893
CATGGCT4443550.083.019884
GGCTACG4434250.082.86867
ATGGCTA4477550.082.3390965
TGGCTAC4504650.081.841656
GCTACGA4471000.081.8184748
CTACGAT4467800.081.459459
TACGATG4422900.040.72929810-11
ACGATGG4455050.040.2924210-11
CGATCCG40400.040.0198061
GATGGGG2818850.035.88963712-13
CGATGGG4865900.035.8471712-13
GATGGGA1087950.033.6726412-13
ATGGGGA930300.033.20262514-15
ATGGGGC581150.032.99664314-15
ATGGGGT980250.032.60392814-15
ATGGGGG609300.031.23305714-15
GATGGGT516900.029.1692812-13
Produced by FastQC (version 0.11.5)