FastQCFastQC Report
Thu 20 Oct 2022
X3-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameX3-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17435505
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA499390.2864212995264548No Hit
CGACATGGCTACGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245540.14082758142078478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG5356600.086.743711
GACATGG5497650.084.756712
ACATGGC5501400.084.698083
CATGGCT5600350.083.415594
GGCTACG5600150.083.149177
ATGGCTA5642600.082.71525
TGGCTAC5667900.082.3459856
GCTACGA5647850.082.12328
CTACGAT5643800.081.808219
TACGATG5586900.040.90932510-11
ACGATGG5617750.040.5257110-11
CGATCCG52750.036.8872031
GATGGGG3546600.036.80182312-13
CGATGGG6066900.036.60087612-13
GATGGGA1297250.035.0040912-13
ATGGGGA1147150.034.71481714-15
ATGGGGC736750.034.3433314-15
ATGGGGT1199950.033.83749414-15
ATGGGGG748100.032.96785414-15
GCTACGG25200.030.9603188
Produced by FastQC (version 0.11.5)