FastQCFastQC Report
Thu 20 Oct 2022
X2-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameX2-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190502
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34110.28651778829434976No Hit
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA13920.1169254650559176No Hit
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13220.11104559253155392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG279200.091.1045761
GACATGG287900.088.661682
ACATGGC288200.088.520473
GGCTACG293050.086.959227
CATGGCT294700.086.8710564
ATGGCTA295050.086.688365
GCTACGA295150.086.069798
TGGCTAC297550.086.038996
CTACGAT294750.085.740129
TACGATG290800.042.8624510-11
ACGATGG291550.042.76024610-11
CGATGGG310900.039.56979812-13
GATGGGG213700.039.3132912-13
ATGGGGA76800.036.62695314-15
ATGGGGT70950.035.70542514-15
ATGGGGG57450.035.46475214-15
GATGGGA59850.035.29908412-13
ATGGGGC28550.034.90017714-15
CGATCCG4250.032.0705871
TGGGGTA23700.031.92826814-15
Produced by FastQC (version 0.11.5)