FastQCFastQC Report
Thu 20 Oct 2022
X2-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameX2-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213909
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28040.23098930809475832No Hit
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA12510.1030555008653861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG307000.090.75441
GACATGG315300.088.708212
ACATGGC317250.088.1629643
CATGGCT321550.087.232474
GGCTACG322100.086.645757
ATGGCTA323950.086.484645
TGGCTAC326550.085.796056
GCTACGA324350.085.667958
CTACGAT323050.085.430749
ACGATGG319100.042.64760210-11
TACGATG319900.042.629110-11
CGATGGG338450.039.73718312-13
GATGGGG237550.039.5508312-13
GCTACGT2200.038.4545488
ATGGGGA88200.037.22165714-15
ATGGGGT74800.036.78943614-15
ATGGGGG63650.036.07148414-15
GATGGGA62950.035.3899912-13
CGATCCG4300.034.9767461
ATGGGGC31400.033.6783414-15
Produced by FastQC (version 0.11.5)