FastQCFastQC Report
Thu 20 Oct 2022
X1-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameX1-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13972841
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA433270.3100801046830777No Hit
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA333380.2385914217445114No Hit
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190630.13642894812873058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG4815750.087.9958651
GACATGG4942050.085.923922
ACATGGC4970550.085.43223
CATGGCT5037850.084.49154
ATGGCTA5090700.083.530325
GGCTACG5073650.083.434927
TGGCTAC5132800.082.825966
GCTACGA5100600.082.600628
CTACGAT5090300.082.3365559
TACGATG5046800.041.15806210-11
ACGATGG5052700.041.03093310-11
GATGGGG3105850.039.3027412-13
CGATGGG5206950.038.8924912-13
ATGGGGA1038000.037.1607914-15
GATGGGA1098650.036.98953212-13
ATGGGGT1072150.036.86046614-15
GCTACGG22300.036.672658
ATGGGGC592750.036.21636614-15
ATGGGGG623150.035.51304214-15
GCTACGT35150.034.2304428
Produced by FastQC (version 0.11.5)