FastQCFastQC Report
Thu 20 Oct 2022
X1-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameX1-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15733028
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA525120.33376918924952015No Hit
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA426180.2708823755986451No Hit
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA232530.14779735979621977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG5687950.087.504391
GACATGG5835100.085.6094062
ACATGGC5870800.085.0856253
CATGGCT5963500.084.034124
ATGGCTA6016350.083.208445
GGCTACG5988250.083.2033167
TGGCTAC6059800.082.609496
GCTACGA6018700.082.4052058
CTACGAT6006400.082.173319
TACGATG5958600.041.0506610-11
ACGATGG5958850.040.9487710-11
GATGGGG3623100.039.6952912-13
CGATGGG6104300.039.08569312-13
ATGGGGA1192200.037.68436414-15
ATGGGGT1253000.037.4968514-15
GATGGGA1245450.037.19205512-13
ATGGGGC691300.037.06697514-15
GCTACGG24500.036.640828
ATGGGGG716500.036.10439714-15
CGTCATG34400.035.933141
Produced by FastQC (version 0.11.5)