FastQCFastQC Report
Thu 20 Oct 2022
DM3-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM3-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17767196
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT349230.19655887175444006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATGG867800.079.799262
ATGGCTA883150.078.513385
CATGGCT888050.078.20724
TGGCTAC888050.078.0960546
CGACATG897800.077.096121
GGCTACG906950.076.302777
ACATGGC934300.074.275393
GCTACGA934550.073.4257058
CTACGAT974850.068.794489
TACGATG953600.034.3406610-11
ACGATGG936550.034.25572610-11
CGATGGG1110000.028.48157512-13
GATGGGG572600.027.51379612-13
GATGGGA457200.026.85640712-13
ATGGGGG92300.023.04171214-15
ATGGGGA366000.022.86434414-15
CGCTTGG69950.021.9714073
GGGGTTA91550.020.38121216-17
CCGCTTG76100.020.1340332
GGGTTAG93250.020.06005316-17
Produced by FastQC (version 0.11.5)