FastQCFastQC Report
Thu 20 Oct 2022
DM3-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM3-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24013441
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT361570.15056984128180548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATGG1211850.079.300992
CATGGCT1229750.078.364554
ATGGCTA1235850.077.8370365
TGGCTAC1235750.077.8129046
CGACATG1249300.076.829751
GGCTACG1254150.076.5026557
ACATGGC1299200.074.056113
GCTACGA1291200.073.7068568
CTACGAT1356550.068.8014459
TACGATG1320550.034.3686710-11
ACGATGG1304600.034.25567210-11
CGATGGG1520150.028.91296812-13
GATGGGG767200.028.3059812-13
GATGGGA611750.027.95798912-13
ATGGGGG128450.025.32035614-15
ATGGGGA491850.023.86550914-15
CGCTTGG89500.021.583243
TGGGAGT133300.020.69692214-15
ATGGGAT270550.020.64664614-15
GGGGAGT107400.020.19599516-17
Produced by FastQC (version 0.11.5)