FastQCFastQC Report
Thu 20 Oct 2022
DM2-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM2-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12842808
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT140450.10936081891125368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTA481750.078.2439045
TGGCTAC489800.077.025116
CGACATG487700.076.990561
CATGGCT493700.076.473774
GACATGG494750.076.102472
GGCTACG495500.076.015757
GCTACGA504250.073.9603358
ACATGGC522350.072.198333
CTACGAT536150.068.2711949
ACGATGG500650.035.09158310-11
TACGATG517450.034.5335810-11
CGATGGG549400.031.46450612-13
GATGGGG284950.030.57185412-13
GATGGGA236100.029.3127912-13
ATGGGGG50700.024.19526514-15
ATGGGGA202800.024.07938814-15
GGGTTAG37050.021.37516816-17
GGGGAGT53350.021.01124616-17
ATGGGGC44200.020.04411914-15
ATGGGAT111950.019.73202514-15
Produced by FastQC (version 0.11.5)