FastQCFastQC Report
Thu 20 Oct 2022
DM2-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM2-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15272213
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTA607400.078.539685
TGGCTAC618200.077.121966
CATGGCT620400.076.977274
GACATGG625950.075.964382
CGACATG624450.075.793091
GGCTACG630450.075.5190667
GCTACGA644350.073.481498
ACATGGC652500.073.039083
CTACGAT685900.067.844739
ACGATGG636850.035.1254610-11
TACGATG663550.034.2114410-11
CGATGGG703600.031.29548312-13
GATGGGG356050.030.41370612-13
GATGGGA314150.028.79245812-13
ATGGGGA253250.025.27699914-15
ATGGGGG65250.024.67049814-15
GGGGAGT68200.021.29472216-17
TGGGAGT80600.019.82630214-15
GGGTTAG33850.019.438716-17
ATGGGGC55950.019.32082414-15
Produced by FastQC (version 0.11.5)