FastQCFastQC Report
Thu 20 Oct 2022
DM1-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM1-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17198155
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTA685300.077.773245
TGGCTAC693100.076.911566
GACATGG699000.076.181692
CGACATG701650.075.786791
GGCTACG703800.075.615387
CATGGCT709950.075.20534
GCTACGA727250.072.744178
ACATGGC734250.072.601163
CTACGAT777650.066.905429
ACGATGG722100.034.70163310-11
TACGATG744000.034.20450210-11
CGATGGG832000.029.71394212-13
GATGGGG424050.029.260712-13
GATGGGA352100.027.87162812-13
ATGGGGA298950.023.55109614-15
ATGGGGG80250.023.28037314-15
TGGGAGT95950.020.8916114-15
GGGGAGT75650.020.31592816-17
GGGAGTA39300.019.19465616-17
GGGTTAG48600.019.14814816-17
Produced by FastQC (version 0.11.5)