FastQCFastQC Report
Thu 20 Oct 2022
DM1-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDM1-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15008058
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT187050.12463304712708333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTA692550.079.5107965
GACATGG699750.078.6322252
TGGCTAC701150.078.522136
CGACATG701450.078.187041
CATGGCT712700.077.4210744
GGCTACG711150.077.232947
GCTACGA725850.075.073088
ACATGGC735400.074.922633
CTACGAT768750.069.770939
ACGATGG723100.035.83985510-11
TACGATG738000.035.58434710-11
CGATGGG807500.031.6631612-13
GATGGGG414300.031.0894312-13
GATGGGA337300.029.61013812-13
ATGGGGG77200.025.96567514-15
ATGGGGA276000.025.40724614-15
GGGTTAG46650.022.71918516-17
GGGGAGT66300.022.68476716-17
ATGGGAT149900.021.25817114-15
GGGGTTA53100.021.06591216-17
Produced by FastQC (version 0.11.5)