FastQCFastQC Report
Thu 20 Oct 2022
DF3-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDF3-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9777183
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGGCGGTCGAGTGCCTCACAGTGTATCAAGGGT330730.33826716754713504No Hit
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT225310.23044469966451483No Hit
CGACATGGCTACGATGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCAA185200.1894206132788964No Hit
CGACATGGCTACGATGGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCA175560.17956092260930373No Hit
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122540.12533262392654407No Hit
CGACATGGCTACGATGTGAACCACAGGAAGCGCAAGTCCGGCACTGTCGG116300.11895041751801107No Hit
CGACATGGCTACGATGGGAGTACTCAAGTCATCGTTCTCTAGTTCAGAGT110470.11298755479978231No Hit
CGACATGGCTACGATGGGAATTGAATTTGCTAAGTCAATCATCTACAAAA99920.10219712569561192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCT2633000.090.476344
ACATGGC2630600.090.32843
CGACATG2636800.089.834381
GACATGG2663100.089.227812
ATGGCTA2680700.088.798045
GCTACGA2721950.086.4232258
TGGCTAC2773800.085.8633656
GGCTACG2773250.085.773627
CTACGAT2716250.085.452199
TACGATG2653850.042.85760510-11
ACGATGG2623900.042.7610610-11
GATGGGG1610050.038.0235712-13
CGATGGG2903000.038.01851712-13
GATGGGA897600.037.85238612-13
ATGGGGG328450.037.60572414-15
ATGGGGA746150.037.11686314-15
TGGGGAA240250.036.25993714-15
ATGGGGC237900.035.65994314-15
TGGGGAG225650.035.47130614-15
GGGGAGT142050.034.7578316-17